Abstract: Mobile Bay is a large estuary located in the southwest corner of Alabama, which connects to the Gulf of Mexico. Mobile Harbor contains the only port in the state that supports ocean-going vessels. Some of the larger vessels calling on the port experience transit delays and limited cargo capacity, so a study was conducted by the US Army Corps of Engineers, Mobile District (CESAM), and the Alabama State Port Authority to investigate channel improvements. In 2017, the US Army Engineer Research and Development Center (ERDC) assisted CESAM in screening proposed deepening and widening alternatives in Mobile Bay by completing a Feasibility Level Ship Simulation (FLSS) study using the ERDC Ship/Tow Simulator. These lower-resolution databases from the FLSS study were used as a foundation to complete a more robust navigation study in 2020 to test the proposed modifications to Mobile Harbor. During this study, three main areas were focused on: a bend easing, a passing lane, and a turning basin. Testing of the proposed design was evaluated over the course of 2 weeks with eight pilots. Assessment of the proposed modifications was accomplished through analysis of ship simulations completed by experienced local pilots, discussions, track plots, run sheets, and final pilot surveys.