Abstract: Contaminants serve as health risks to recreational water, potable water, and marine life that result in undocumented effects on population exposure. In many areas of the world, the concern lies in contaminated drinking water, which would immediately effect social and economic order. As research advances for innovative solutions, the deployment of automated systems for source water monitoring could reduce the risk of exposure. Water quality monitoring typically involves sample collection and analyses that are performed in a laboratory setting. These results are normally presented after an 18−48 hr period. This report details the prototyped Autonomous QUerying And PATHogen threat agent sensor (AQUA PATH) geoenabled system that is able to detect the presence/absence of pathogenic bacteria indicators in source waters and report these values in the field, in less than 30 minutes. The AQUA PATH system establishes rapid field data collection and reports assessment of source waters bacterial loads at near shore inner coastal locations, which makes a leap forward compared to current presence/absence tests standards established by the EPA.