Abstract: In 2019, the US Army Engineer Research and Development Center, Coastal and Hydraulics Laboratory (CHL), Ship/Tow Simulator (STS), was used to perform a navigation study assisting the US Army Corps of Engineers, Jacksonville District. The study evaluates channel improvements to allow larger containerships to call at the Port of Miami. This study also evaluates improvements associated with the proposed cruise terminals. This study was conducted at the CHL real-time STS. Real-time refers to the fact that model time uses a 1:1 ratio to prototype time. In addition, real world environmental forces were simulated and acted upon the modeled ships during the study. These forces included currents, wind, bathymetry, and bank effects. Simulations for the project improvements were conducted at CHL for 3 weeks in August 2019 and 1 week in November 2019. Seven Biscayne Bay pilots participated in the validation and testing exercises. The design vessels include the MSC Daniela (14,000 twenty-foot equivalent unit [TEU]) container ship, the Majestic Maersk (18,000 TEU) container ship, the Allure of the Seas cruise ship, the Disney Dream cruise ship, and the M/S Paradise cruise ship. Results, in the form of track plots and pilot questionnaires, were reviewed to develop conclusions and recommendations.