Abstract: Currently, no standardized method exists for the analysis of insensitive munitions (IM) in environmental matrices such as water, soils, and tis-sues. However, standardized methods, such as United States Environmental Protection Agency (EPA) 8330B, exist for legacy munitions for water and soil matrices. The lack of standardized methods for IM analysis leads researchers to use a wide variety of incomplete and overlapping analytical methodologies. The overall project’s first phase, Strategic Environmental Research and Development Program (SERDP) Environmental Restoration (ER)–2722, was to develop and optimize methods to address these methodological gaps by creating analytical methods for simultaneous analysis of IM and legacy munitions in water, soil, and tissue matrices. The main objective of the current project phase, Environmental Security Technology Certification Program (ESTCP) ER19-5078, is to build upon the previous work in phase one and to focus on the validation of the newly developed methods. Synergizing with the main objective of the overall project, the methods were validated and submitted to the EPA for inclusion as a possible addendum to EPA 8330B.